25-HYDROXY VITAMIN D (D2+D3)

Vitamin D – 25 Hydroxy (D2+D3) Test 

Test Overview

Sample Type	Blood
Reports Delivery	6 Hrs
Price/Cost	₹1800
Number of Tests Included	1

We often overlook the important role of minerals and vitamins, particularly Vitamin D. Vitamin D, also called the ‘Sunshine Vitamin’, is extremely essential for maintaining optimal bone health and immunity. Typically our body produces vitamin D when exposed to sunlight. However, due to more time spent indoors and intense pollution, there is limited sun exposure, resulting in vitamin D deficiency. This is where the Vitamin D – 25 Hydroxy (D2+D3) Test becomes invaluable.

The top Vitamin D – 25 Hydroxy (D2+D3) tests are available in Hyderabad, Bangalore, Gurgaon, Noida, Chennai.

This test offers a simplified yet reliable method to confirm adequate intake of this crucial vitamin, thereby protecting you from potential health complications associated with both deficiency and excess levels of vitamin D. Let’s know more about this test in the sections below.

Vitamin D – 25 Hydroxy (D2+D3) Test Price/Cost 

Vitamin D test price is an important factor when deciding the place to get tested. Understand the price structure and make an informed decision that aligns with your budgetary needs. Consider your options wisely when scheduling a Vitamin D test as your health is a worthwhile investment. The vitamin D Test price can vary depending on the following factors:

1. Demand for the test

• Vitamin D test prices may vary based on demand and availability in different areas.
• Regions with a higher demand may have slightly higher vitamin D test prices.

2. Inclusions

• Vitamin D test price may cover components like the doctor’s fee, lab charges, and convenience fees for home sample collection if you opt for it.
• It’s essential to review the inclusions in the price before making payment.

3. Quality of the lab

• High-end laboratories may charge more due to their superior testing standards and credibility.
• Check the certification of the lab before booking a vitamin D test.

4. Discounts and Deals

• Healthcare providers near you may offer discounts or deals, such as free sample collection, which can reduce the overall vitamin D test price.
• Check for any available offers before booking the test.

At Apollo 24|7 the price of the tests is competitive and backed by our commitment towards providing quality healthcare services. Remember to consult with your doctor regarding any questions or concerns about this test.

All You Need to Know About Vitamin D – 25 Hydroxy (D2+D3) Test

The Vitamin D – 25 Hydroxy (D2+D3) Test is a simple blood test that measures the level of 25 hydroxy vitamin d2 d3 in your bloodstream. Doctors recommend this test when they suspect a patient may have a deficiency or excess of Vitamin D.

1. What is the Vitamin D – 25 Hydroxy (d2+d3) Test?

The Vitamin D – 25 Hydroxy (d2+d3) Test evaluates the combined levels of vitamin D2 and vitamin D3, providing a thorough assessment of vitamin D. This test helps doctors diagnose vitamin D deficiency. Vitamin D deficiency can have significant implications on bone health, immune function, and overall well-being.

Further, the test can help identify excessive vitamin D levels, which may lead to toxicity and adverse health effects. Based on the test results, doctors can recommend appropriate interventions such as dietary changes, supplements, or exposure to sunlight to optimise vitamin D levels and promote overall health.

2. Components of Vitamin D – 25 Hydroxy (d2+d3) Test

Here are the components of the Vitamin D test :

• Vitamin D2: Vitamin D2 is also known as ergocalciferol. It is obtained from plant-based sources and dietary supplements. It is less commonly found in the human body as compared to vitamin D3.
• Vitamin D3: Vitamin D3 is also called cholecalciferol. It is produced in the skin upon exposure to sunlight and is also found in certain foods such as fatty fish and fortified dairy products. It is the most biologically active form of vitamin D.
• Vitamin D 25 Hydroxy: Vitamin D 25-hydroxy, or 25(OH)D, is a compound produced by the liver as it metabolises both vitamin D2 and vitamin D3. It serves as the main circulating form of vitamin D in the blood. Notably, it is considered the best indicator of vitamin D status in the body.

3. Who Requires a Vitamin D – 25 Hydroxy (d2+d3) Test?

Conditions that may require you to get Vitamin D – 25 Hydroxy (d2+d3) Test include:

• Age: Older adults, usually over 65, may experience reduced skin synthesis of vitamin D due to age-related changes in skin structure and low sun exposure. They may also have lower dietary intake or impaired absorption of the vitamin, which can increase their risk of deficiency.
• Skin tone: People with darker skin tones have low vitamin D synthesis because melanin inhibits sunlight absorption. This requires prolonged sun exposure and often leads to higher susceptibility to deficiency, particularly in regions with limited sunlight.
• Body weight: Obesity can reduce circulating vitamin D levels as excess body fat can sequester the vitamin, lowering its bioavailability despite sufficient intake or sun exposure.
• Weight loss surgeries: Surgeries like gastric bypass can disrupt nutrient absorption, including vitamin D, due to changes in the digestive system, increasing deficiency risk.
• Limited sun exposure: Whether due to lifestyle, occupation, or environmental factors like long winters or high pollution, less than normal exposure to sunlight can result in inadequate vitamin D production.
• Medical conditions: Conditions like celiac disease, inflammatory bowel disease, Crohn’s disease, or cystic fibrosis can impair nutrient absorption, including vitamin D. This increases the risk of deficiency risk and necessitates regular monitoring of vitamin D levels.

For individuals with these risk factors, undergoing a Vitamin D test, near their place, is recommended. It can provide valuable insights into their vitamin D status and guide appropriate interventions, such as dietary modifications, supplementation, or increased sun exposure, to optimise their vitamin D levels and mitigate the risk of deficiency-related health complications.

4. When should you take the Vitamin D – 25 Hydroxy (d2+d3) Test?

Here are some symptoms that might indicate you need to take the Vitamin D Test :

• Constant Fatigue and Tiredness: Since vitamin D plays a crucial role in energy metabolism, deficiency may lead to feelings of fatigue and lethargy, even with adequate rest.
• Continuous Bone or Back Pain: Vitamin D is essential to maintain bone health and calcium metabolism. Hence, deficiency can contribute to pain in the back and joints along with osteoporosis, and increased susceptibility to fractures.
• Frequent Pain or Weakness in Muscles: Vitamin D is required for muscle contraction and strength regulation. Vitamin D deficiency can impair muscle function and cause muscle pain, weakness, or cramps.
• Depression: Vitamin D receptors are present in areas of the brain associated with mood regulation. Low vitamin D levels have been linked to a high risk of depression and mood disorders.
• Persistent Hair Fall: Vitamin D deficiency may contribute to hair loss or thinning. This is because vitamin D receptors are present in hair follicles, therefore adequate levels are necessary for hair growth and maintenance.
• Menstrual Problems: Research reveals that there is a relationship between vitamin D status and menstrual cycle disorders, including irregular cycles, oligomenorrhoea (infrequent menstruation), and amenorrhoea (absence of menstruation).
• Frequent infections: Vitamin D deficiency is associated with poor immune function and frequent infections.

If you experience any of these symptoms, or if you belong to the high-risk category for vitamin D deficiency, you should discuss your concerns with your doctor and consider undergoing the Vitamin D – 25 Hydroxy (d2+d3) Test. However, it’s important to note that these symptoms can also be indicative of other health conditions, so a comprehensive evaluation by a medical professional is recommended before confirming the diagnosis.

Explaining the Range & Parameters of Vitamin D – 25 Hydroxy (D2+D3) Test

Below are the normal, low, and high ranges of the parameters measured by the Vitamin D – 25 Hydroxy (D2+D3) test :

Vitamin D2 Range

• Normal range: 25-80 ng/mL.
• Low: Below 20 ng/mL
• High: Above 100 ng/mL

Vitamin D3 Range:

• Normal: Between 30 and 60 ng/mL
• Low: Below 20 ng/mL
• High: Above 80 ng/mL

25 Hydroxy Vitamin D Range:

• Normal: Between 30 and 100 ng/mL
• Low: Below 20 ng/mL
• High: Above 80 ng/mL

How to Read Vitamin D – 25 Hydroxy (D2+D3) Test Report?

Depending on your reports of vitamin D test, your vitamin D levels can either be low, high or normal. Let’s understand them.

1. What does the 25-Hydroxy Vitamin D deficiency indicate?

Low levels of vitamin D may indicate inadequate exposure to sunlight, insufficient intake of the vitamin through diet or trouble absorbing it from food. Notably, vitamin D deficiency can lead to bone disorders such as rickets in children and osteomalacia in adults.

If your vitamin D – 25 Hydroxy (D2+D3) test report shows low vitamin D levels, it could be because of the following reasons:

• Low Sun Exposure: Low or limited exposure to sunlight hampers the skin’s ability to produce vitamin D.
• Pigmented Skin: Higher levels of melanin in darker skin tones reduce vitamin D synthesis.
• Poor Diet: Inadequate consumption of vitamin D-rich foods like fatty fish or fortified dairy products.
• Medical Conditions: Liver or kidney diseases, such as metabolic-associated fatty liver disease (MAFLD) and chronic kidney disease (CKD), impair vitamin D metabolism or utilisation.
• Improper Absorption: Conditions like Crohn’s disease or celiac disease hinder vitamin D absorption from the intestines.
• Medications: Certain drugs such as cholesterol-lowering agents (statins, cholestyramine and colestipol), antiseizure medications (valproic acid), glucocorticoids, orlistat, thiazides, and metformin, interfere with vitamin D metabolism.

2. What happens when you have excess Vitamin D in your blood?

Too much vitamin D in the blood can result in hypervitaminosis D, which is commonly associated with consuming excessive amounts of vitamin D through supplements. Here are some potential implications:

• Hypercalcemia: The excess vitamin D can cause an increase in the level of calcium in your blood, leading to a condition known as hypercalcemia.
• Kidney damage: High calcium levels in your blood can lead to kidney stones or even kidney damage in severe cases.

3. Tests that may be recommended in case of low Vitamin D levels

If you have low levels of vitamin D, your doctor may suggest additional tests such as:

• Calcium and Phosphorus tests: These tests help understand the absorption of these minerals.
• Parathyroid hormone (PTH) test: PTH helps regulate calcium levels in your body. A high PTH level could indicate a problem with your parathyroid glands.
• 1,25-Dihydroxyvitamin D test: The vitamin D test measures the active form of vitamin D in your body and can help ascertain the cause of a deficiency or excess.

4. Tests that may be recommended in case of high Vitamin D levels

In case of high vitamin D levels, your doctor may order tests to assess kidney function and calcium levels in the blood, including:

• Kidney function panel: KFT or Kidney function panel includes a set of tests that measure different chemicals and substances in your urine and blood to evaluate how well your kidneys are working.
• Urine calcium: The urine calcium test measures the amount of calcium in your urine over 24 hours.
• Parathyroid hormone (PTH) level: High PTH levels with high vitamin D levels could indicate a problem with your parathyroid glands.

5. Which doctor to consult for Vitamin D – 25 Hydroxy (d2+d3) Test?

Depending on your specific health concerns and symptoms, you may be asked to consult a general physician, orthopaedician, or endocrinologist for the Vitamin D – 25 Hydroxy (d2+d3) Test.

Why is the Vitamin D – 25 Hydroxy (D2+D3) Test Important?

The Vitamin D – 25 Hydroxy (d2+d3) Test plays a crucial role in monitoring an individual’s overall health.

1. Importance of the Vitamin D – 25 Hydroxy (d2+d3) Test important?

Here’s why the Vitamin D – 25 Hydroxy (d2+d3) Test is important:

• Indicates the status of Vitamin D: This test measures the concentration of 25-hydroxy vitamin D in your blood. Apt levels of vitamin D help maintain strong bones and teeth, while also supporting your immune system.
• Detection of Deficiencies: This test helps detect vitamin D deficiencies, which can otherwise lead to conditions such as osteoporosis or rickets in children. Early detection can help in prompt treatment and management.
• Helps Evaluating Overall Health: The test also helps assess overall health as low vitamin D levels may be linked to various health issues such as thyroid problems and cardiovascular diseases.

2. Which diseases can be diagnosed with the Vitamin D – 25 Hydroxy (d2+d3) Test?

The Vitamin D – 25 Hydroxy (D2+D3) Test serves as a valuable diagnostic tool for various health conditions, offering insights into:

• Bone Disorders: By measuring Vitamin D levels, this test assists in diagnosing bone-related diseases such as osteoporosis and rickets. Adequate Vitamin D is essential for calcium absorption and bone mineralisation, making it integral to bone health assessment.
• Risk of Other Conditions: Beyond bone health, the Vitamin D – 25 Hydroxy (d2+d3) Test aids in assessing the risk of developing other conditions at an early stage. Low Vitamin D levels have been associated with an increased risk of multiple sclerosis, rheumatoid arthritis, and certain types of cancer, for example, prostate, multiple myeloma, colorectal, and breast cancer. However, further testing is required before confirming any diagnosis.
• For Monitoring Treatment Effectiveness: For those, who are taking vitamin D supplements or treatment, the vitamin D test serves as a valuable tool to monitor treatment effectiveness. Regular monitoring allows healthcare professionals to adjust treatment plans, ensuring optimal vitamin D levels and therapeutic outcomes.

By aiding with early diagnosis, risk assessment, and treatment monitoring, the vitamin D test plays a crucial role in promoting proactive healthcare management and improving patient outcomes.

3. Is Vitamin D – 25 Hydroxy (d2+d3) Test important for pregnant women?

Regular testing is important for pregnant women due to several reasons, some of which include:

• Healthy Pregnancy: Adequate Vitamin D levels are needed for a healthy pregnancy. Deficiencies in expectant mothers can lead to complications such as gestational diabetes and preterm birth.
• Prevents Preeclampsia: Regular testing helps prevent conditions like preeclampsia, a pregnancy complication characterised by high blood pressure and low vitamin D levels.
• Supports Baby’s Development: Adequate Vitamin D levels help ensure the baby’s healthy bone development.

4. What should be the frequency of the Vitamin D test?

The frequency of the vitamin D test depends on numerous factors, as follows:

• Current Health Status: If you are diagnosed with a vitamin D deficiency or at risk of developing it, your doctor might suggest getting tested more frequently.
• Age and Lifestyle: Elderly people, those with limited sun exposure, and individuals with certain dietary restrictions may need regular testing.
• As Advised by the Doctor: For most people, getting tested annually or as advised by your doctor is sufficient to keep a check on Vitamin D levels.

Food Rich In Vitamin D & Vitamin D Supplements

A balanced diet plays a pivotal role in maintaining the right Vitamin D levels.

1. How does your diet help maintain Vitamin D levels?

Numerous foods naturally contain Vitamin D, whereas some are fortified with this essential nutrient. Here’s a list of some superfoods rich in Vitamin D:

• Fatty Fish: Fish such as salmon, mackerel, and tuna are among the richest natural sources of Vitamin D.
• Fish Liver Oils: Cod liver oil is an excellent source of Vitamin D. It can be a beneficial dietary supplement for those who don’t consume much fish or reside in places with less sunlight.
• Liver: This organ meat is not only rich in Vitamin D but also an excellent source of iron and protein.
• Egg Yolks: If you do not like seafood or meat, eggs can be your go-to source of Vitamin D.
• Cheese: Certain types of cheese, like cheddar and Swiss, contain small amounts of Vitamin D.
• Fortified Foods: Many everyday foods are fortified with Vitamin D, including milk, certain brands of orange juice, yoghurt, margarine, and breakfast cereals.

2. How to get Vitamin D supplements?

Apart from food sources, you might consider taking vitamin D supplements if you’re deficient or at risk of deficiency. There are two forms:

• Vitamin D2 (Ergocalciferol): This type is often found in fortified foods and most over-the-counter vitamin supplements.
• Vitamin D3 (Cholecalciferol): This form is found in supplements and foods like fatty fish and egg yolks. Many experts believe that this is the more effective of the two.

How to Prepare for Vitamin D – 25 Hydroxy (D2+D3) Test?

Getting ready for the vitamin D test is simple. Here’s how you can prepare:

• Consult with a doctor: Consult with your doctor about any specific instructions that might be relevant to your health condition before going for a test.
• Fasting or dietary restrictions: Generally, there’s no need for fasting or any special dietary restrictions before the test.

1. How is the Vitamin D – 25 Hydroxy (d2+d3) Test done?

Vitamin D test involves drawing blood, usually from a vein in your arm. The collected blood sample is then dispatched to a laboratory for detailed analysis.

2. Can a Vitamin D test be taken at any time of the day?

You can take the Vitamin D test at any time of the day. However, it is crucial to note the time of sample collection as Vitamin D levels can fluctuate throughout the day.

3. Are there any risks involved with the Vitamin D – 25 Hydroxy (d2+d3) Test?

Risks associated with vitamin D tests are minimal to none. There may be slight pain or bruising at the site where the blood was drawn. Apart from this minor discomfort, the test is generally safe for most people.

4. Can Vitamin D – 25 Hydroxy (d2+d3) Test be taken at home?

You can book a home sample collection for Vitamin D – 25 Hydroxy (d2+d3) Test and provide the blood sample in the comfort of your home. Please note that you cannot perform the test by yourself at home.

5. What alternative tests can be recommended with the Vitamin D test?

In some cases, based on your Vitamin D – 25 Hydroxy (d2+d3) Test results, your doctor may recommend further tests as follows:

• If the vitamin D levels are low, additional tests such as calcium, phosphorus, Alkaline Phosphatase (ALP), and Parathyroid Hormones (PTH) might be recommended.
• In the case of high Vitamin D levels, your doctor may order X-rays or other imaging tests to assess the impact on your bones.

Remember, maintaining an optimal Vitamin D level in your body is essential for your overall health. This simple blood test can be a step towards ensuring better health and well-being.

How to Book Vitamin D – 25 Hydroxy (d2+d3) Test Online?

Scheduling your Vitamin D – 25 Hydroxy (d2+d3) Test online with Apollo 24|7 is straightforward straightforward and involves a few simple steps:

1. Steps to book the Vitamin D – 25 Hydroxy (d2+d3) Test on Apollo 24|7

• Visit the Apollo 24|7 Website or App: Begin by navigating to the official Apollo 24|7 website or download their user-friendly app on your mobile.
• Search for the Test: In the search box provided, type “Vitamin D – 25 Hydroxy (d2+d3) Test.” This will lead you to the test’s specific page.
• Choose a Convenient Time and Location: After selecting the test, you can choose a suitable date and time for blood sample collection. Some centres offer free sample collection. You can opt for home sample collection if you prefer not to visit a diagnostic centre.
• Complete the Booking: Finalise your booking by providing the required details and confirming your appointment.
• Wait for Confirmation: After the successful completion of your booking, you will receive a confirmation message from Apollo 24|7.

Though booking online tests is convenient and safe, ensure you follow your doctor’s advice about preparation before the test.

2. How to download the Vitamin D – 25 Hydroxy (d2+d3) Test report online on Apollo 24|7?

Once the sample collection of Vitamin D – 25 Hydroxy (d2+d3) Test is completed, follow these steps:

• Check for Notification: After your test results are ready, you’ll receive a notification from Apollo 24|7.
• Visit Your Account: Log into your Apollo 24|7 account on the website or app.
• Navigate to the Report Section: Go to the ‘Reports’ section where all your test results are stored.
• Select the Test: Find and click on the Vitamin D – 25 Hydroxy (d2+d3) Test to view your report.
• Download Your Report: There will be an option available for you to download and save your report for future reference.

This report is crucial in assessing your vitamin D levels, however, it’s always a good idea to discuss your results with your doctor for accurate understanding and interpretation.